Related Experiment Video
Updated: Aug 25, 2026
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Published on: July 7, 2013
[The fibroblast primary cell culture by the split-thickness skin slide technique]
Yu-ming Zhao1, Jin Zuo, Rui Cao
1Department of the Aesthetic & Plastic Surgery on the Face and Neck, Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100041, China.
Objective:
To acquire lots of cell to culture during the primary cell culture.
Method:
We take the split-thickness skin slide technique to acquire the dissociated fibroblast cell in two big-ear rats.
Results:
The cell number is above 10(6) from 1 cm x 2 cm split-thickness skin slide and the technique is simple, economic, effectve.
Conclusion:
We think this way is better than other methods, and should be adopted in the primary cell culture, especially in fibroblast transplantation by injection.
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