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[Determination of the structure of the main component of an antibiotic complex produced by Streptomyces griseolus
A E Kabanov1, A N Danilenko, I A Spiridonova
1I. M. Sechenov Moscow Medical Academy, N. M. Emmanuel Research Institute of Biochemical Physics, Russian Academy of Sciences, National Research Centre of Antibiotics, Moscow.
Abstract:
In the programme for screening antibiotics with antifungal and immunosuppressing activity a culture of Streptomyces griseolus 182 was isolated. The culture produced a complex of oligomycin structure antibiotics. Individual components of the complex were isolated by HPLC. The complex was shown to contain three components at a ratio of 80:15:5. The findings were compared with the physico-chemical characteristics of the described antibiotics. On the basis of the analysis it was concluded that fraction 2 of the antibiotic complex 182 could be oligomycin A. A detailed comparative investigation of oligomycin A and component 2 with HPLC, FMR, IR spectroscopy and mass spectrometry revealed their identity. The other two components were shown to be oligomycins B and C.
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