Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evaluation of the E test by using selected gram-positive bacteria.

J H Ngui-Yen1, E A Bryce, C Porter

  • 1Medical Microbiology, Vancouver General Hospital, British Columbia, Canada.

Journal of Clinical Microbiology
|August 1, 1992
PubMed
Summary

The E test accurately determines minimum inhibitory concentrations (MICs) for gram-positive bacteria, offering a reliable, reproducible, and cost-effective alternative to standard methods for antibiotic susceptibility testing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Pilot Randomised Controlled Trial to Compare 3D Printed Versus Conventionally Fabricated Complete Dentures.

The European journal of prosthodontics and restorative dentistry·2025
Same author

Newborn screening follow-up for very long-chain acyl-CoA dehydrogenase deficiency in Colorado: Working towards a standardized protocol.

Molecular genetics and metabolism·2025
Same author

Correction for Williams et al., "<i>In vitro</i> comparison of methods for sampling copper-based antimicrobial surfaces".

Microbiology spectrum·2024
Same author

<i>In vitro</i> comparison of methods for sampling copper-based antimicrobial surfaces.

Microbiology spectrum·2023
Same author

Patient perspectives on nipple-areola complex micropigmentation during the COVID-19 pandemic.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2022
Same author

Evaluating the antimicrobial activity of copper surfaces against Pseudomonas aeruginosa and Staphylococcus aureus 1 year after use in a microbiology laboratory.

The Journal of hospital infection·2022

Area of Science:

  • Microbiology
  • Clinical Laboratory Science
  • Antimicrobial Resistance

Background:

  • Accurate determination of antibiotic minimum inhibitory concentrations (MICs) is crucial for effective treatment of bacterial infections.
  • Standard reference methods for MIC determination can be time-consuming and costly.
  • The E test offers a potential alternative for MIC determination.

Purpose of the Study:

  • To evaluate the accuracy, reproducibility, and cost-effectiveness of the E test compared to standard reference methods for MIC determination.
  • To assess the E test's performance against various gram-positive bacteria, including Streptococcus pneumoniae, Enterococcus faecium, and Staphylococcus aureus (both oxacillin-sensitive and resistant).

Main Methods:

  • The E test was compared with National Committee for Clinical Laboratory Standards (NCCLS) recommended reference methods.

Related Experiment Videos

  • MICs were determined for four antibiotics against 208 clinical isolates of gram-positive bacteria.
  • Evaluation included MIC accuracy, reproducibility testing, and cost analysis.

    • Main Results:

    • High agreement rates were observed between the E test and reference methods for most bacteria-antibiotic combinations (e.g., 94% for S. pneumoniae/penicillin G, 92% for E. faecium/ampicillin, 100% for E. faecium/vancomycin).
    • Use of salt-supplemented Mueller-Hinton agar improved oxacillin E test accuracy with staphylococci (85-100% agreement).
    • The E test demonstrated excellent reproducibility and was more cost-effective than microdilution panels for testing fewer than three antibiotics.

    Conclusions:

    • The E test is a simple, reliable, reproducible, and cost-effective method for MIC determination in clinical microbiology laboratories.
    • It provides accurate MIC values for a range of gram-positive organisms, aiding in appropriate antibiotic selection.
    • The E test is a valuable tool for antibiotic susceptibility testing, particularly in resource-limited settings or for routine testing of a limited number of agents.