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Related Experiment Videos

Effect of direct-fed microbials on rumen microbial fermentation.

S A Martin1, D J Nisbet

  • 1Department of Animal and Dairy Science, University of Georgia, Athens 30602.

Journal of Dairy Science
|June 1, 1992
PubMed
Summary

Direct-fed microbials, including Aspergillus oryzae and Saccharomyces cerevisiae, can enhance ruminant nutrition by stimulating key bacterial growth and fermentation. These microbial supplements appear to provide factors that improve lactate utilization in the rumen.

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Area of Science:

  • Rumen microbiology
  • Animal nutrition
  • Fermentation science

Background:

  • Direct-fed microbials (DFMs) like Aspergillus oryzae and Saccharomyces cerevisiae are used in ruminant diets.
  • Research on DFM efficacy in ruminants shows variable results regarding feed utilization and performance.
  • In vitro studies on ruminal microorganisms also yield inconsistent findings on DFM effects.

Purpose of the Study:

  • To investigate the effects of Aspergillus oryzae fermentation extract and Saccharomyces cerevisiae on the growth and metabolism of Selenomonas ruminantium.
  • To determine if DFMs provide soluble factors that stimulate lactate utilization by ruminal bacteria.
  • To explore the potential role of malate in mediating these microbial responses.

Main Methods:

  • In vitro incubation of Selenomonas ruminantium with A. oryzae fermentation extract and S. cerevisiae culture.

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  • Measurement of bacterial growth, lactate uptake, and fermentation end-product production (acetate, propionate, succinate, VFA).
  • Analysis of potential stimulatory compounds, such as malate, in the DFM products.

    • Main Results:

    • Both A. oryzae and S. cerevisiae markedly increased Selenomonas ruminantium growth in lactate medium.
    • Lactate uptake by S. ruminantium whole cells was significantly enhanced by the DFM products.
    • Production of acetate, propionate, succinate, total volatile fatty acids (VFAs), and cell yield increased with DFM treatment.
    • Evidence suggests malate content in DFMs may contribute to the observed stimulation of lactate utilization.

    Conclusions:

    • Aspergillus oryzae and Saccharomyces cerevisiae provide soluble factors that stimulate lactate utilization by Selenomonas ruminantium.
    • These DFMs can enhance key metabolic functions of important ruminal bacteria, potentially improving overall rumen function.
    • Malate is implicated as a potential active component responsible for the observed stimulatory effects of these DFMs.