Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput microfluidic mixing and multiparametric cell sorting for bioactive compound screening.

Susan M Young1, Mark S Curry, John T Ransom

  • 1Cytometry, Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, USA.

Journal of Biomolecular Screening
|March 10, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bone Marrow Mesenchymal Stem Cell Extracellular Vesicle Treatment of Respiratory Failure from COVID-19: Endpoint Analysis of Expanded Access Safety Trial.

Stem cell reviews and reports·2026
Same author

Acute wood smoke exposure is associated with cell-specific hippocampal transcriptomic responses in an accelerated ovarian failure mouse model.

bioRxiv : the preprint server for biology·2026
Same author

The Concise Guide to PHARMACOLOGY 2025/26: G protein-coupled receptors.

British journal of pharmacology·2025
Same author

Treatment of severe traumatic brain injury with human bone marrow mesenchymal stem cell extracellular vesicles: a case report.

Brain injury·2025
Same author

Bone marrow mesenchymal stem cell-derived extracellular vesicle infusion for amyotrophic lateral sclerosis.

Neurodegenerative disease management·2024
Same author

GPER expression prevents estrogen-induced urinary retention in obese mice.

The Journal of steroid biochemistry and molecular biology·2024

This study introduces an automated flow cytometry system for high-throughput screening of bioactive compounds. The HyperCyt system, combined with cell sorting, efficiently identifies highly responsive cells for drug discovery.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Pharmacology

Background:

  • Automated sample handling is crucial for high-throughput screening.
  • Flow cytometry enables detailed cell analysis.
  • Previous HyperCyt configurations analyzed cell responses but lacked sorting integration.

Purpose of the Study:

  • To expand HyperCyt capabilities for high-throughput bioactive compound screening using automated cell sorting.
  • To assess the system's sensitivity and carryover performance.
  • To isolate and characterize highly responsive cell subsets.

Main Methods:

  • Utilized the HyperCyt automated sample handling system with an autosampler and air bubbles for sample separation.
  • Integrated automated cell sorting with flow cytometry for screening.

Related Experiment Videos

  • Sampled peptide ligands from a 96-well plate, mixed with fluorescently labeled U937 cells, and analyzed Ca(2+) responses.
  • Sorted highly responsive cells for further analysis.

    • Main Results:

    • Screened peptide ligands at 3 reactions per minute, analyzing ~10,000 cells per reaction.
    • Detected cell Ca(2+) responses to peptide concentrations as low as 10(-11) M.
    • Demonstrated no detectable carryover between samples up to 10(-7) M peptide.
    • Sort-purified cells showed enhanced sensitivity and sustained responses.

    Conclusions:

    • The integrated HyperCyt and cell sorting system enables high-throughput screening of bioactive compounds.
    • The system accurately detects low-concentration responses with minimal carryover.
    • This technology facilitates the isolation of sensitive cell subsets, promising efficient screening of millions of compound interactions daily.