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Updated: Aug 25, 2026
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
Published on: June 8, 2022
Fluorescence-labeled sphingosines as substrates of sphingosine kinases 1 and 2
Peter Ettmayer1, Andreas Billich, Thomas Baumruker
1Novartis Institute for Biomedical Research, Brunnerstrasse 59, Vienna A-1235, Austria. peter.ettmayer@pharma.novartis.com
Abstract:
Fluorescently labeled d-erythro-sphingosines have been successfully synthesized in 9 and 12 steps starting from commercially available Garner aldehyde. Determining the influence of the nature, position and linkage of the label on the in vitro phosphorylation rate by sphingosine kinases 1 and 2 resulted in the identification of a pyrene- and a NBD-labeled sphingosine which are both phosphorylated with efficiency comparable to the natural substrate.
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