Photodynamic therapy upregulates expression of Mac-1 and generation of leukotriene B(4) by human polymorphonuclear leukocytes
Wataru Kobayashi1, Qiang Liu, Tomoh Matsumiya
1Department of Oral and Maxillofacial Surgery, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan. wako@cc.hirosaki-u.ac.jp
Abstract:
This study investigated the expression of Mac-1 (CD11b/CD18) and generation of leukotriene B(4) (LTB(4)) by polymorphonuclear leukocytes (PMNs) in response to Mono-L-aspartyl chlorine 6 (NPe6) mediated photodynamic therapy (PDT). PDT was carried out using a laser diode at a light dose of 10 J/cm(2) and wavelength of 664 nm. Expression of Mac-1 was significantly increased about 3-fold by PDT compared with that of Npe6 exposure. The increase in the expression of Mac-1 was not inhibited by 5-lipoxygenase inhibitor (AA861). On the other hand, generation of LTB(4) by PMNs was increased by incubation with NPe6 alone and further promoted by PDT and that was significantly inhibited by AA861. These results suggest that PDT can induce PMNs to express extensively Mac-1 and generate a certain amount of LTB(4) that may play an important cytocidal role in the treatment of oral cancer.
Insights
Photodynamic therapy (PDT) significantly increases Mac-1 expression and leukotriene B(4) generation in polymorphonuclear leukocytes (PMNs). These effects suggest a role for PDT-induced PMN activity in oral cancer treatment.
Area of Science:
- Biomedical Sciences
- Oncology
- Immunology
Background:
- Photodynamic therapy (PDT) is an emerging cancer treatment modality.
- Polymorphonuclear leukocytes (PMNs) play a crucial role in the immune response.
- Mac-1 and leukotriene B(4) (LTB(4)) are key mediators in inflammatory processes.
Purpose of the Study:
- To investigate the effect of N-aspartylchlorin e6 (NPe6)-mediated PDT on Mac-1 expression and LTB(4) generation by PMNs.
- To explore the potential role of PDT-induced PMN activation in oral cancer therapy.
Main Methods:
- PMNs were exposed to NPe6 and subsequently treated with PDT (10 J/cm(2), 664 nm).
- Mac-1 expression was quantified using flow cytometry.
- LTB(4) generation was measured using enzyme-linked immunosorbent assay (ELISA).
- The effect of 5-lipoxygenase inhibitor (AA861) on Mac-1 expression and LTB(4) generation was evaluated.
Main Results:
- PDT significantly increased Mac-1 expression on PMNs by approximately 3-fold compared to NPe6 exposure alone.
- The increase in Mac-1 expression was not affected by the 5-lipoxygenase inhibitor AA861.
- LTB(4) generation was enhanced by NPe6 incubation and further promoted by PDT.
- PDT-induced LTB(4) generation was significantly inhibited by AA861.
Conclusions:
- PDT effectively induces Mac-1 expression on PMNs.
- PDT enhances LTB(4) generation by PMNs, an effect mediated by 5-lipoxygenase.
- PDT-induced Mac-1 expression and LTB(4) generation in PMNs may contribute to the cytocidal activity observed in oral cancer treatment.
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