Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences between ATP-mediated cytotoxicity and cell-mediated cytotoxicity.

R K Avery1, K J Bleier, M S Pasternack

  • 1Infectious Disease Unit Children's and Medical Services, Massachusetts General Hospital, Boston 02114.

Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1992
PubMed
Summary

Extracellular ATP can cause cell lysis, similar to cytotoxic T lymphocytes (CTLs). However, ATP-mediated lysis differs as it does not induce apoptosis and resistant cell lines can be generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virus-Specific T-Cell Therapy for Prophylaxis and Treatment of Cytomegalovirus Infections After Transplantation: a Scoping Review.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2025
Same author

Epidemiology and outcomes of Clostridium difficile infection in allogeneic hematopoietic cell and lung transplant recipients.

Transplant infectious disease : an official journal of the Transplantation Society·2018
Same author

Induction Immunosuppression and Clinical Outcomes in Kidney Transplant Recipients Infected With Human Immunodeficiency Virus.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Invasive aspergillosis before HCT: safe to proceed?

Bone marrow transplantation·2015
Same author

Solid organ transplantation from hepatitis B virus-positive donors: consensus guidelines for recipient management.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

KIR and HLA interactions are associated with control of primary CMV infection in solid organ transplant recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2013

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Extracellular adenosine triphosphate (ATP) is increasingly recognized for its role in cell-mediated cytotoxicity.
  • Investigating the mechanisms of ATP-mediated lysis is crucial for understanding immune responses and cellular damage.

Purpose of the Study:

  • To compare the similarities and differences between extracellular ATP-mediated lysis and cytotoxic T lymphocyte (CTL)-mediated lysis.
  • To characterize the ATP-mediated lysis of EL-4 cells and identify distinct cellular responses.

Main Methods:

  • Studied ATP-mediated lysis of EL-4 cells, noting time and concentration dependence.
  • Compared kinetics of ATP- and cell-mediated lysis.
  • Generated ATP-resistant EL-4 cell lines by continuous exposure to high ATP concentrations.

Related Experiment Videos

Main Results:

  • ATP-mediated lysis of EL-4 cells occurred within 4–6 hours and was concentration-dependent.
  • ATP-mediated lysis did not induce chromosomal degradation (apoptosis), unlike CTL-mediated lysis.
  • Derived EL-4 cell lines resistant to ATP but still susceptible to CTL-mediated lysis.

Conclusions:

  • Extracellular ATP-mediated lysis and CTL-mediated lysis share kinetic similarities but differ in apoptotic induction.
  • Identified distinct cellular mechanisms underlying ATP and CTL cytotoxicity.
  • Developed ATP-resistant cell lines, offering a model to differentiate these cytotoxic pathways.