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Related Experiment Videos

Biosynthesis and secretion of complement component (C3) by activated human polymorphonuclear leukocytes.

M Botto1, D Lissandrini, C Sorio

  • 1Institute of General Pathology, University of Verona, Italy.

Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1992
PubMed
Summary

Human polymorphonuclear leukocytes (PMN) synthesize complement component 3 (C3) when activated by LPS or TNF-alpha. This secreted C3 is functional and may play a role in host defense during inflammation.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Complement System

Background:

  • Human polymorphonuclear leukocytes (PMN) express complement receptors CR1 and CR3.
  • The synthesis of complement component 3 (C3) by PMN was previously uncharacterized.

Purpose of the Study:

  • To investigate whether human PMN can synthesize C3, particularly upon activation.
  • To determine the functional capacity of PMN-derived C3.

Main Methods:

  • Northern blot analysis of PMN RNA after stimulation with LPS and cytokines (IFN-gamma, TNF-alpha, IL-1).
  • ELISA to quantify C3 secretion.
  • SDS-PAGE and [35S]methionine labeling to analyze C3 protein.
  • [14C]methylamine incorporation and autolytic cleavage assays to assess C3 functionality.

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Main Results:

  • PMN express C3 mRNA and secrete functional C3 protein when stimulated with LPS or TNF-alpha.
  • IFN-gamma and IL-1 induced a transient increase in C3 mRNA but not significant C3 secretion.
  • PMN-derived C3 contains an intact thiolester bond, indicating functionality.

Conclusions:

  • Human PMN synthesize and secrete functional C3 in response to specific inflammatory stimuli (LPS, TNF-alpha).
  • This capability suggests a novel role for PMN in host defense mechanisms at inflammatory sites.