Sub-second isoelectric focusing in free flow using a microfluidic device
Yi Xu1, Chao-Xuan Zhang, Dirk Janasek
1Imperial College London, Chemistry Department, Exhibition Road, London, UK SW7 2AZ.
Lab on a Chip
|March 10, 2004
Abstract:
Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.


