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[The development and ultrastructure study of acellular submandibular gland scaffold]
Chun-fu Deng1, Yue Liu, Xue-xin Tan
1Department of Oral and Maxillofacial Surgery, First Clinical Hospital of China Medical University, Shenyang 110001, China.
Purpose:
To develop nature acellular submandibular gland scaffold materials and observe its ultrastructures.
Methods:
The fresh submandibular glands of SD rats were cell-extracted by chemical detergent and observed with transmission and scanning electron microscope.
Results:
Submandibular gland cells were dissolved and destroyed under transmission electron microscope. Normal submandibular gland cells were ball, but the shape of cells disappeared after cell-extraction under scanning electron microscope. Collagen fibers were arranged as net structure.
Conclusions:
Submandibular gland cells disappeared and the arrangement of collagen fibres were normal by Triton X-100 cell-extraction.