Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase synthesis of DOTA-peptides.

Luis M De León-Rodriguez1, Zoltan Kovacs, Gregg R Dieckmann

  • 1Department of Chemistry, University of Texas at Dallas, P.O. Box 830688, Richardson, TX 75083, USA.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|March 10, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "The rate of lactate production from glucose in hearts is not altered by per-deuteration of glucose" [J. Magn. Reson. 284 (2017) 86-93].

Journal of magnetic resonance (San Diego, Calif. : 1997)·2025
Same author

Detection of Two Different Inner-Sphere Solvent Species in a Eu<sup>3+</sup>-Based ParaCEST Agent Dissolved in a Mixture of Methanol and Water: The Impact of Solvents on Exchange Dynamics.

Inorganic chemistry·2025
Same author

APT imaging of hepatocellular carcinoma signals an effective therapeutic response in advance of tumor shrinkage.

Hepatic oncology·2025
Same author

Impact of dietary zinc on stimulated zinc secretion MRI in the healthy and malignant mouse prostate.

Npj imaging·2024
Same author

Enhanced Oligopeptide and Free Tryptophan Release from Chickpea and Lentil Proteins: A Comparative Study of Enzymatic Modification with Bromelain, Ficin, and Papain.

Plants (Basel, Switzerland)·2024
Same author

Combining NIR spectroscopy with chemometrics for discriminating naturally ripened banana and calcium carbide ripened banana.

NPJ science of food·2024

Researchers developed a versatile method to link DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) to amino acids for peptide synthesis. This allows precise DOTA placement in peptides for potential targeted therapies.

Area of Science:

  • Chemical Synthesis
  • Peptide Chemistry
  • Radiopharmaceutical Development

Background:

  • Peptide-based therapeutics and diagnostics often require conjugation with chelating agents like DOTA.
  • Controlled and versatile methods for incorporating DOTA into peptides are crucial for developing targeted agents.

Purpose of the Study:

  • To describe a general synthetic route for DOTA-linked amino acid derivatives.
  • To demonstrate the utility of these derivatives in solid-phase peptide synthesis.
  • To enable the controlled positioning of DOTA within peptide sequences for screening.

Main Methods:

  • Synthesis of two DOTA-linked N-Fmoc amino acids (DOTA-F and DOTA-K).
  • Solid-phase peptide synthesis using standard HBTU activation and pre-activated C-pentafluorophenyl esters.

Related Experiment Videos

  • Preparation of DOTA-conjugated 12-mer peptides for binding studies.

    • Main Results:

    • Successful synthesis of DOTA-linked amino acid derivatives.
    • Demonstrated utility in solid-phase peptide synthesis, with specific conditions for chain extension beyond DOTA insertion.
    • Preparation of three GdDOTA-12-mers with DOTA at different positions and comparison of their binding to Gal-80.

    Conclusions:

    • A versatile and controlled method for introducing DOTA into any position within a peptide sequence has been established.
    • This methodology facilitates the screening of DOTA-linked peptide libraries for optimized targeting properties.
    • The approach holds potential for developing novel peptide-based radiopharmaceuticals and imaging agents.