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Matrix metalloproteinase 9 expression is coordinately modulated by the KRE-M9 and

Takashi Kobayashi1, Jiro Kishimoto, Shunji Hattori

  • 1Department of Dermatology/Clinical Biology of Extracellular Matrix, Chiba University School of Medicine, Chiba, Japan. tkobayas@faculty.chiba-u.jp

The Journal of Investigative Dermatology
|March 11, 2004
PubMed

Insights

Matrix metalloproteinase 9 (MMP-9) transcription is regulated by the KRE-M9 element, which acts as a repressor, and the activator protein 1 (AP-1) binding to the TRE. This coordinated control is vital in skin and connective tissues.

Area of Science:

  • Molecular biology
  • Cell biology
  • Biochemistry

Background:

  • Matrix metalloproteinase 9 (MMP-9) plays a role in various pathophysiologic conditions.
  • Understanding MMP-9 transcriptional regulation is crucial for studying diseases involving this enzyme.

Purpose of the Study:

  • To investigate the transcriptional regulation mechanism of matrix metalloproteinase 9 (MMP-9).
  • To analyze the function of the KRE-M9 element and its binding protein in MMP-9 gene expression.

Main Methods:

  • Analysis of MMP-9 promoter activity in keratinocytes and HT1080 fibrosarcoma cells.
  • Investigation of transcription factor binding to the KRE-M9 and TRE elements within the MMP-9 promoter.
  • Assessment of protein levels in relation to cellular differentiation.

Main Results:

  • The KRE-M9 element is essential for MMP-9 transcription, and its binding protein acts as a transcriptional repressor.
  • Activator protein 1 (AP-1) proteins activate MMP-9 transcription by binding to the adjacent TRE element.
  • The KRE-M9 binding protein functions as differentiation repressing factor 1 (DRF-1), with its levels decreasing during differentiation.

Conclusions:

  • Coordinated modulation of MMP-9 transcription via TRE and KRE-M9 elements is important in epidermal and mesenchymal tissues.
  • Findings provide insight into the molecular mechanisms underlying MMP-9 involvement in various pathophysiologic conditions.

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