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Updated: Aug 25, 2026
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
Published on: March 23, 2011
[Study on changes of neuron apoptosis after focal cerebral ischemia/reperfusion in the aged rats]
Jian-sheng Li1, Xiao-qiao Ren, Ke Liu
1Geriatrics Department of Henan College of Traditional Chinese Medicine, Zhengzhou 450003, Henan, China.
Objective:
To study ultrastructured change and neuron apoptosis after focal cerebral ischemia/reperfusion (I/R) in the aged rats.
Methods:
The aged SD rats (20-21 months) and the young (4-5 months) were subjected to 3 hours of middle cerebral artery occulsion with the intraluminal filament technique, followed by reperfusion for 3 hours, 6 hours, 12 hours, 24 hours, 72 hours. The ultrastructure of brain, infarct zone and neuron apoptosis were observed.
Results:
The cerebral infarct zone increased in the aged rats with ischemia for 3 hours and I/R for 12 hours compared with those in the young. The brain injury was obviously deteriorated with I/R time longer, which was serious in the aged than that in the young. The neuron apoptosis increase with I/R time longer, and showed earlier and lasted longer in the aged.
Conclusion:
The cerebral injury is serious and neuron apoptosis increase shows early and lasts longer.
