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Two-photon-induced fluorescence.

P R Callis1

  • 1Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. callis@chemistry.montana.edu

Annual Review of Physical Chemistry
|January 1, 1997
PubMed
Summary

This review covers nonresonant two-photon electronic spectroscopy of polyatomic molecules since 1979. It highlights spectral patterns, substituent effects, and applications in biological molecules using two-photon fluorescence anisotropy.

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Area of Science:

  • Molecular Spectroscopy
  • Quantum Chemistry
  • Biophysical Chemistry

Background:

  • Nonresonant two-photon electronic spectroscopy provides unique insights into molecular electronic structure.
  • Since 1979, significant advancements have been made in understanding polyatomic molecules.
  • Aromatic hydrocarbons and biological molecules are key subjects of study.

Purpose of the Study:

  • To review the progress in nonresonant two-photon electronic spectroscopy since 1979.
  • To emphasize patterns in excitation spectra and the influence of molecular properties.
  • To discuss theoretical predictions and emerging applications.

Main Methods:

  • Analysis of two-photon fluorescence, ionization, and optoacoustic excitation spectra.
  • Application of pseudoparity rules to understand spectral patterns.
  • Investigation of vibronic coupling and substituent effects using quantum chemical methods.

Main Results:

  • Identified patterns in two-photon spectra of aromatic hydrocarbons.
  • Demonstrated the impact of vibrations and substitution on spectral features.
  • Highlighted the utility of two-photon-induced fluorescence anisotropy in biological studies.

Conclusions:

  • Nonresonant two-photon spectroscopy is a powerful tool for molecular characterization.
  • Theoretical calculations accurately predict molecular properties relevant to spectroscopy.
  • The field continues to evolve with new applications and higher-order techniques.

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