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Updated: Aug 25, 2026

Dynamic Electrochemical Measurement of Chloride Ions
Published on: February 5, 2016
Electrochemical study of hydrochlorothiazide and its determination in urine and tablets
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria, El-Messalah 21521, Egypt. amarazak@hotmail.com
Abstract:
A voltammetric study of hydrochlorothiazide (HCTZ) at glassy carbon electrode was carried out. The drug in Britton-Robinson buffer (pH 3.3) is oxidized at +1040mV, giving rise to a well-defined peak. Cyclic voltammetric study indicates that the oxidation process is irreversible and diffusion-controlled. A sensitive, simple and time-saving differential pulse anodic voltammetric procedure has been developed. The procedure has been applied for the drug determination in human urine with no prior extraction and in commercial tablets. The proposed method has been validated. The limit of detection for the standard solution was 5.0ngml(-1) and for the drug in urine was 14.0ngml(-1). The results obtained from the analysis of commercial tablets were compared statistically with those obtained from the USP 26 high-performance liquid chromatographic (HPLC) method.
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