Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dextran sulfate provides a quantitative and quick microarray hybridization reaction.

Wei-Chi Ku1, Wai Kwan Lau, Yu-Tien Tseng

  • 1Department of Research and Development, U-Vision Biotech, Inc., Taipei, Taiwan, ROC.

Biochemical and Biophysical Research Communications
|March 12, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of a novel Dysferlin microdeletion knock-in mouse model mimicking muscular dystrophy-like pathology.

Scientific reports·2026
Same author

Timescale-dependent Phosphoproteomic Remodeling and Motility-associated Adaptation under Chronic Cabozantinib Exposure in Renal Cell Carcinoma.

Cancer genomics & proteomics·2026
Same author

Factors associated with beyond-therapeutic serum voriconazole trough level among critically ill patients with invasive fungal infections.

BMC infectious diseases·2025
Same author

Establishment of High-Throughput Screening Assay using 384-Well Plate for Identification of Potent Antioxidants from Malaysian Local Plants Repository and Phytochemical Profile of <i>Tetracera Scandens</i>.

Tropical life sciences research·2025
Same author

Collagen proteins, thrombospondin 1 and lumican are differentially expressed across breast cancer subtypes by functional proteomics from core needle biopsy samples of Taiwanese breast cancer.

Biochemistry and biophysics reports·2025
Same author

Overview of Cellular Therapeutics Clinical Trials: Advances, Challenges, and Future Directions.

International journal of molecular sciences·2025
Same journal

SMURF1-mediated EFEMP1 ubiquitination reverses the resistance of HCC cells to sorafenib by promoting ferroptosis.

Biochemical and biophysical research communications·2026
Same journal

Development and validation of HSP90AA1 as a risk gene in a HIF-1α pathway-related prognostic signature for hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

Quercetin suppresses TGF-β1-induced proliferation and migration of vascular smooth muscle cells via the Smad2/3/MMP-9 signaling axis.

Biochemical and biophysical research communications·2026
Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026
Same journal

COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma.

Biochemical and biophysical research communications·2026
Same journal

Tumor budding in colorectal cancer: partial EMT, microenvironmental remodeling, and metastatic competence.

Biochemical and biophysical research communications·2026
See all related articles

This study introduces a novel hybridization solution for microarrays that significantly enhances reaction speed and signal intensity. This breakthrough enables more sensitive detection of rare transcripts, improving genomic analysis accuracy.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Microarray technology accelerates genomics research but requires improvements in hybridization efficiency.
  • Current microarray hybridization protocols take 16 hours or overnight using high salt buffers (e.g., SSC).
  • The quantitative nature of microarray hybridization has been under-investigated.

Purpose of the Study:

  • To develop an improved hybridization solution for microarrays.
  • To enhance reaction kinetics and signal output.
  • To achieve quantitative hybridization and detect rare transcripts.

Main Methods:

  • A novel hybridization solution containing dextran sulfate was formulated.
  • The solution's effect on hybridization kinetics and signal intensity was tested on cDNA and oligonucleotide microarrays.

Related Experiment Videos

  • Quantitative analysis of hybridization signals was performed.
  • The impact on background signals and signal-to-noise ratio was assessed.

    • Main Results:

    • The dextran sulfate solution increased hybridization signals 4-fold for cDNA and 29-fold for oligonucleotide microarrays.
    • The hybridization reaction became quantitative, with signals proportional to transcript abundance.
    • Rare transcripts were detectable with slightly longer reaction times.
    • Background signals did not increase, leading to higher signal-to-noise ratios.

    Conclusions:

    • Dextran sulfate-containing hybridization solutions significantly accelerate microarray reactions.
    • This approach enables quantitative hybridization and sensitive detection of low-abundance transcripts.
    • The improved method enhances microarray performance without increasing background noise.