Expression of the chemokine receptor Cxcr4 mRNA during mouse brain development

The expression of Cxcr4 mRNA that encodes the receptor for the chemokine Sdf1 was studied during mouse brain development using in situ hybridization, from E9.5 to maturity at P21. At embryonic stages, expression is prominent in ventricular zones of stem cell proliferation. This abates during the postnatal period in parallel to the depopulation of ventricular zones. In addition, the Cxcr4 gene is expressed in some differentiating neuronal populations at E12.5, E14.5 and E17.5, such as scattered cells in the reticular formation, cranial nerve nuclei, peripheral ganglia, cerebellar external granule cells, zona incerta, ventral lateral geniculate thalamic nuclei, olfactory glomerular layer, hippocampal primordium and telencephalic preplate. High levels of expression are detected in preplate derivatives in all sectors of the marginal zone (MZ) of the telencephalic vesicles, including Cajal-Retzius (CR) cells, other MZ cells and subplate neurons. Cxcr4 expression is progressively downregulated postnatally, but remains significantly associated in the adult with Bergman glia in the cerebellum, the subgranular layer of the dentate gyrus, and the olfactory glomerular layer. In contrast, expression of Sdf1 mRNA is confined to the meninges and, in embryos, to the telencephalic intermediate zone. This expression pattern suggests that Sdf1 and its receptor Cxcr4 may exert trophic influences on precursor cell proliferation and some neuronal targets that remain to be identified and studied further.