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[A study of fluoride and dexamethasone affecting the proliferation in osteoblast]
1Department of Oral Medicine, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Objective:
Investigate the effects of low dose of fluoride and dexamethasone on the proliferation of MC3T3-E1 osteoblast cell line in vitro, which was for determining the possible effects of fluoride and dexamthasone affecting the development and differentiation of osteoblast.
Methods:
Observe the growing shape of MC3T3-E1 osteoblast cultured in vitro, and investigate the effects of low dose of fluoride and dexamethasone on the proliferation of osteoblast by cell count.
Results:
MC3T3-E1 osteoblast grew stably. The cells in D group increased obviously in the early stage of the cell culture, however the cells in F group had no tendency of increasing during the whole culture.
Conclusion:
In vitro low doses of dexamethasone may promote the proloferation of osteoblast precursors,however,low dose of fluoride has no effect on promoting proliferation of MC3T3-E1 osteoblast.
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