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[Study on plant tissue culture of Cornus officinalis]
Jian-ping Xue1, Ai-min Zhang, Yue-hui Wang
1Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China. xuejp2000@yahoo.com.cn
Objective:
To set up the optimums of tissue culture for Cornus officinalis.
Method:
A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone.
Result And Conclusion:
The WPM medium with 6-BA 2.0 mg.L-1 + ZT 0.1 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg.L-1 + ZT 0.1 mg.L-1 + GA3 0.5 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2 MS medium with IBA 1.0 mg.L-1 + 6-BA 0.1 mg.L-1.
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02:57Plant Tissue Culture
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