Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Murine Nasal Lavage Fluid Collection without Blood Contamination05:12

Murine Nasal Lavage Fluid Collection without Blood Contamination

1.7K
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory...
1.7K
Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome03:40

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome

1.1K
OSTSS leads to pooled inflammatory mediators in the tear film, causing symptoms like epiphora and discomfort. Here, we present a case where therapeutic nasolacrimal lavage every 2 months resolved epiphora and itchiness, suggesting its efficacy as a therapeutic intervention for OSTSS. Additionally, we report subjective improvements in symptoms in 3 additional...
1.1K
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

880
Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
880
Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber08:47

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

2.9K
A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental...
2.9K
Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians06:49

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians

12.0K
Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment...
12.0K
Intranasal Administration of CNS Therapeutics to Awake Mice07:15

Intranasal Administration of CNS Therapeutics to Awake Mice

61.4K
A method to intranasally administer drugs to awake mice for the purpose of targeting the brain is described. This method allows for repeat dosing over long periods using intranasal administration of drug without anesthesia, and nose-to-brain delivery with minimal systemic...
61.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

ppGpp regulates transcription elongation via direct and indirect inputs to RNA polymerase pausing and nucleotide addition.

bioRxiv : the preprint server for biology·2026
Same author

RNAP-seq: <i>in vitro</i> genome-scale transcription reveals preferential RNA polymerase pausing on <i>Clostridioides difficile</i> antisense DNA.

bioRxiv : the preprint server for biology·2026
Same author

RNA polymerase inhibitors reveal active-site motions essential for the nucleotide-addition cycle.

bioRxiv : the preprint server for biology·2026
Same author

Cell-free genomics reveals fundamental regulatory principles of the Mycobacterium tuberculosis transcription cycle.

Molecular cell·2026
Same author

Harnessing evolution: leveraging bacterial isoprenoid pathway diversity toward improved bioengineering strategies.

Journal of bacteriology·2026

Related Experiment Video

Updated: Jan 7, 2026

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
03:40

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome

Published on: April 25, 2025

1.1K

Active-site dynamics in RNA polymerases.

Robert Landick1

  • 1Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.

Cell
|March 16, 2004
PubMed
Summary

New research reveals bacteriophage T7 RNA polymerase uses a nucleotide-addition cycle similar to DNA polymerases. This finding offers insights into complex RNA polymerases in other organisms.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Bacteriophage T7 RNA polymerase is a key enzyme in viral gene expression.
  • Understanding RNA polymerase mechanisms is crucial for deciphering cellular transcription.

Discussion:

  • Crystal structures reveal active-site conformational changes during nucleotide addition.
  • The observed mechanism resembles that of DNA polymerases, suggesting conserved principles.

Key Insights:

  • Identified a nucleotide-addition cycle in T7 RNA polymerase.
  • Demonstrated similarity to DNA polymerase mechanisms.
  • Provided structural basis for RNA synthesis.

Outlook:

  • Proposes new hypotheses for complex multisubunit RNA polymerases.

More Related Videos

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

880
Intranasal Administration of CNS Therapeutics to Awake Mice
07:15

Intranasal Administration of CNS Therapeutics to Awake Mice

Published on: April 8, 2013

61.4K

Related Experiment Videos

Last Updated: Jan 7, 2026

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
03:40

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome

Published on: April 25, 2025

1.1K
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

880
Intranasal Administration of CNS Therapeutics to Awake Mice
07:15

Intranasal Administration of CNS Therapeutics to Awake Mice

Published on: April 8, 2013

61.4K
  • Potential implications for understanding transcription in free-living organisms.
  • Opens avenues for structural studies of other RNA polymerases.