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Updated: Aug 17, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment
1Institut de Biologie Structurale - Jean-Pierre Ebel, UMR5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France. Bernhard.Brutscher@ibs.fr
Abstract:
A simple and general method is presented to simplify multi-dimensional NMR spectra of isotope-labeled bio-molecules. The approach is based on band-selective Hadamard-type frequency encoding, which disperses the correlation peaks into different sub-spectra. This makes it possible to apply low-dimensionality-based NMR techniques to larger molecular systems. Here we demonstrate the use of band-selective Hadamard frequency labeling for fast protein resonance assignment, based on our recently proposed suite of 2D experiments (Bersch et al., 2003).
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