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Updated: Aug 25, 2026
Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
[Expression of GBD gene of Streptococcus mutans glucan binding protein A in mammalian cells]
Ling-yun Su1, Bu-ling Wu, Fu-yang Li
1Dept. of Oral Medicine, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To evaluate the expression of recombinant plasmid pcDNA3.1/GBD of glucan binding protein of Streptococcus mutans in mammalian cells COS-7.
Methods:
Eukaryotic plasmid carrying encoding gene of GBD of Streptococcus mutans gbpA was constructed and the plasmid was introduced into COS-7 cells by Lipofectamine reagent. The transient expressed protein in COS-7 cells was detected by immunochemistry technique.
Results:
The positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pcDNA3.1/GBD. The cells which were transfected with pcDNA3.1 were negative.
Conclusion:
GBD can translate and express in COS-7 cells after transfected with recombinant plasmid pcDNA3.1/GBD. The expressed protein locates in the plasma and the protein is able to combine with anti-GbpA antibody. The expressed protein has the antigenicity and is a candidate gene vaccine.
