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Updated: Aug 25, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
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Determination of shapes and maximums of analyte peaks based on solute mobilities in capillary electrophoresis
Ning Fang1, Eon Ting, David D Y Chen
1Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z1.
Abstract:
In capillary electrophoresis, the relative orders of mobilities of analyte, additive, and the complex formed determine the analyte peak shape in a way similar to the way the binding isotherms determine the peak shapes in chromatography. The three mobilities allow six possible orders; each produces a characteristic peak shape in CE. Equations describing the analyte migration in a CE system with the presence of mobility-changing additives can be implemented into computer programs to predict the migration times of the analyte peak maximums, and the predicted migration times agree well with the experimental results.
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