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Published on: October 6, 2014
Expression of Apc2 during mouse development
Hiroki Yamanaka1, Norio Hashimoto, Kumiko Koyama
1Department of Anatomy and Neuroscience, Hyogo College of Medicine, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan. yamanaka@hyo-med.ac.jp
Abstract:
APC2 (previously known as APCL), a molecule closely related to the adenomatous polyposis coli (APC) tumor suppressor, can deplete cytoplasmic beta-catenin, like APC itself. Recently, it has been shown that APC2 may regulate the localization of p53 and the microtubule stability and/or extension. Although it has been reported that APC2 mRNA is expressed in human brain, the anatomical and ontogenic expression patterns remain unclear. The purpose of this study was to investigate the distribution of mouse Apc2 during mouse development. In the adult brain, Apc2 is expressed predominantly in neurons and throughout the brain. Northern blot analysis demonstrated a high level of Apc2 expression in embryonic and early postnatal brain. Ontogenic analysis has indicated that Apc2 is expressed in neural tissue, including the peripheral nervous system. During development of cortex, retina and cerebellum, Apc2 is expressed in post-mitotic cells. These findings suggest that Apc2 may contribute to the development of neuronal cells.
Insights
Adenomatous polyposis coli 2 (APC2) is expressed in the developing mouse brain, particularly in neurons and neural tissues. This suggests APC2 plays a role in neuronal cell development.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- Adenomatous polyposis coli 2 (APC2), related to the APC tumor suppressor, influences beta-catenin levels.
- APC2 may regulate p53 localization and microtubule dynamics.
- APC2's expression pattern in the developing brain is not well understood.
Purpose of the Study:
- To investigate the anatomical and developmental expression of mouse Apc2.
- To understand the role of Apc2 in neural development.
Main Methods:
- Northern blot analysis
- Ontogenic analysis of gene expression in mouse tissues
Main Results:
- Apc2 is predominantly expressed in adult mouse brain neurons.
- High Apc2 expression detected in embryonic and early postnatal brain.
- Apc2 expression observed in neural tissue, including the peripheral nervous system.
- Apc2 found in post-mitotic cells during cortex, retina, and cerebellum development.
Conclusions:
- Apc2 is widely distributed in the developing mouse nervous system.
- Apc2 expression patterns suggest a role in neuronal cell development.
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