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Acute no-effect dose for in ova exposure to C3F7 tagged 5-hydroxytryptophan, a novel probe for investigating neural
Sherry Dingman1, Lara Hurlburt, Craig Branch
1Marist College, Poughkeepsie, NY, USA. Sherry.Dingman@marist.edu
Purpose:
To create a probe for investigating neural development in chicks using magnetic resonance, a C(3)F(7) tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 microg/egg) administered in ova results in 0.5-1.0 microg accumulating in a 250 microL midbrain.
Procedures:
Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development.
Results:
No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [X(2)(obs)=2.30, X(2)(crit) (df=2)=5.99, P>.05].
Conclusion:
The no-effect dose for in ova administration of C(3)F(7) tagged5-HTP is 5 microg/egg on Day 17.
