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Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
08:10

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Comparisons of envelope through 5B sequences of infectious bronchitis coronaviruses indicates recombination occurs in

Judy Elaine Brooks1, Aaron Cameron Rainer, Rebecca Lynn Parr

  • 1Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.

Virus Research
|March 17, 2004
PubMed

Insights

This study sequenced infectious bronchitis virus (IBV) strains, revealing high genetic identity but also recombination events. These findings are crucial for understanding IBV evolution and vaccine development.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Infectious bronchitis virus (IBV) is a significant pathogen in poultry.
  • Understanding IBV genetic diversity and evolution is key for disease control.

Purpose of the Study:

  • To analyze the genetic sequences of US IBV strains.
  • To identify genetic variation and recombination events within IBV.

Main Methods:

  • Sequencing of a 1.78 kb region including E, M, 5a, and 5b genes of six US IBV strains.
  • Comparison of obtained sequences with two additional published IBV strains.
  • Nucleotide sequence analysis to determine genetic identities and detect recombination.

Main Results:

  • Overall nucleotide sequence identities ranged from 90-99% across the analyzed region.
  • The 5b open reading frame (ORF) showed the highest identity (94-99%).
  • Recombination events were detected in the M gene of recent field isolates and potentially in E and M ORFs of laboratory strains.

Conclusions:

  • US IBV strains exhibit high genetic similarity but also evidence of recombination.
  • Recombination events involving vaccine-like strains suggest complex IBV evolution.
  • Findings highlight the need for ongoing genetic surveillance of IBV.

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