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Antigenic secreted proteins from Haemophilus paragallinarum. A 110-kDa putative RTX protein
Erika Mena-Rojas1, Candelario Vázquez Cruz, Sergio Vaca Pacheco
1Centro de Investigaciones en Ciencias Microbiológicas, BUAP, Apdo. Postal 1622, Puebla 72560, Mexico.
Abstract:
Haemophilus paragallinarum is the causal agent of infectious coryza, an economically important disease for the poultry industry. This bacterium secreted proteins of 25-110 kDa during its growth in brain heart infusion, tryptic soy broth, or Luria-Bertani glucose phosphate media, all lacking serum. Some of these proteins were recognized by sera from chickens experimentally infected with H. paragallinarum. A 110-kDa protein was recognized by a serum pool from convalescent-phase pigs naturally infected with Actinobacillus pleuropneumoniae, and also by a rabbit polyclonal serum against Apx I as well as a rabbit serum against Mannheimia haemolytica leukotoxin, suggesting the presence of an RTX-like protein in H. paragallinarum. H. paragallinarum secreted proteins could be important immunogens in the control of infectious coryza.
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