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Related Experiment Videos

ISWI complexes in Saccharomyces cerevisiae.

Jane Mellor1, Antonin Morillon

  • 1Department of Biochemistry, Microbiology Unit, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. jane.mellor@bioch.ox.ac.uk

Biochimica Et Biophysica Acta
|March 17, 2004
PubMed
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The imitation switch (ISWI) chromatin remodeling complexes in yeast regulate gene expression through various mechanisms. These complexes are involved in transcription repression, elongation control, and termination, often interacting with RNA polymerase II.

Area of Science:

  • Chromatin biology
  • Molecular genetics
  • Biochemistry

Background:

  • Imitation switch (ISWI) ATPases are crucial chromatin remodelers found in eukaryotes.
  • ISWI enzymes function within larger protein complexes, where associated proteins determine their cellular roles.
  • Budding yeast, Saccharomyces cerevisiae, possesses two ISWI ATPases, Isw1p and Isw2p, making it a model system for studying ISWI function.

Purpose of the Study:

  • To elucidate the diverse functions of yeast ISWI complexes in gene expression regulation.
  • To understand how different ISWI-containing complexes interact with other cellular machinery.
  • To explore the roles of ISWI complexes in transcription, elongation, and termination.

Main Methods:

  • Leveraging genetic manipulation techniques in Saccharomyces cerevisiae.

Related Experiment Videos

  • Analyzing data from systematic and global screens for ISWI function.
  • Investigating biochemical assays and cellular functions of ISWI complexes.

    • Main Results:

    • Yeast Isw1p and Isw2p complexes are implicated in controlling RNA levels and repressing gene expression.
    • Specific ISWI complexes are involved in displacing transcription machinery, coordinating elongation, and ensuring transcription termination.
    • Phosphorylation of the RNA polymerase II C-terminal domain (CTD) regulates transcription elongation and termination functions of ISWI complexes.

    Conclusions:

    • Yeast ISWI complexes play multifaceted roles in gene expression, including repression, elongation control, and termination.
    • The function of ISWI complexes is modulated by their associated proteins and interactions with RNA polymerase II.
    • Further research is needed to fully characterize novel ISWI complexes and their cooperative mechanisms with other chromatin-modifying enzymes.