Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nociceptin/orphanin FQ modulates human T cell function in vitro.

Paula S Waits1, Wendy M Purcell, Allison J Fulford

  • 1Faculty of Applied Sciences, Centre for Research in Biomedicine, University of the West of England, Coldharbour Lane, Frenchay, Bristol, BS16 1QY, UK.

Journal of Neuroimmunology
|March 17, 2004
PubMed
Summary

Nociceptin/orphanin FQ (N/OFQ) influences human T cell activation, enhancing proliferation and modulating TNF-alpha secretion. It also inhibits re-stimulated T cells by up-regulating CTLA-4, partly via prostaglandin synthesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Teaching Climate and Health in Preventive Medicine Residency Programs: A Survey of Program Directors.

Journal of graduate medical education·2024
Same author

Higher education and the COVID-19 pandemic: navigating disruption using the sustainable development goals.

Discover sustainability·2022
Same author

Spheroid Size Does not Impact Metabolism of the β-blocker Propranolol in 3D Intestinal Fish Model.

Frontiers in pharmacology·2018
Same author

Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper.

Ecotoxicology (London, England)·2017
Same author

Pharmaceutical Metabolism in Fish: Using a 3-D Hepatic In Vitro Model to Assess Clearance.

PloS one·2017
Same author

Correction: Direct Measurements of Oxygen Gradients in Spheroid Culture System Using Electron Parametric Resonance Oximetry.

PloS one·2016

Area of Science:

  • Immunology
  • Neuroendocrinology
  • Cellular Biology

Background:

  • The nociceptin/orphanin FQ (N/OFQ) peptide and its receptor (ORL-1) are widely expressed in immune cells.
  • The precise function of the N/OFQ system in immune responses, particularly T cell activation, remains largely undefined.

Purpose of the Study:

  • To investigate the role of N/OFQ in modulating human T cell activation and function.
  • To elucidate the mechanisms underlying N/OFQ's effects on T cells, including potential involvement of prostaglandins.

Main Methods:

  • Human T cells were treated with varying concentrations of N/OFQ.
  • T cell activation markers (e.g., CD28), proliferation, and TNF-alpha secretion were assessed.
  • CTLA-4 expression and prostaglandin synthesis were analyzed in response to N/OFQ stimulation.

Related Experiment Videos

Main Results:

  • N/OFQ (10(-14)-10(-12) M) upregulated activation markers like CD28, enhancing T cell proliferation and modulating TNF-alpha secretion.
  • On re-stimulated T cells, N/OFQ inhibited proliferation, correlating with increased CTLA-4 expression.
  • N/OFQ was found to induce prostaglandin synthesis, and some of its effects were partly prostaglandin-dependent.

Conclusions:

  • N/OFQ plays a significant modulatory role in human T cell activation and function.
  • The peptide influences T cell responses through mechanisms involving both activation and inhibition, potentially mediated by prostaglandin synthesis.