Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Separation of individual neurons using dielectrophoretic alternative current fields.

Shalini Prasad1, Xuan Zhang, Mo Yang

  • 1Department of Electrical Engineering, University of California Riverside, A 220 Bourns Hall, Riverside, CA 92521, USA.

Journal of Neuroscience Methods
|March 17, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State of mission: Battery management with neural networks and electrochemical AI.

iScience·2025
Same author

Epitaxial Growth Control of Crystalline Morphology and Electronic Transport in InSb Nanowires: Competition Between Axial and Radial Growth Modes.

Nanomaterials (Basel, Switzerland)·2025
Same author

Charging the Future with Pioneering MXenes: Scalable 2D Materials for Next-Generation Batteries.

Nanomaterials (Basel, Switzerland)·2025
Same author

Eco-friendly layered carbide synthesis from renewable precursors for energy storage applications.

iScience·2025
Same author

Powering the Future: Unveiling the Potential of Na, K, and Mg Solid-State Batteries.

Nanomaterials (Basel, Switzerland)·2025
Same author

Renewable saccharide-derived porous carbon foams with metal particles for CNT and graphene substrates in electrochemical applications.

iScience·2025

This study introduces a novel microelectrode array system for isolating and positioning individual neurons using dielectrophoretic traps. This method enables precise recording of neuronal electrical activity for neuroscience research.

Area of Science:

  • Neuroscience
  • Bioengineering
  • Electrical Engineering

Background:

  • Understanding neuronal network dynamics is crucial for neuroscience.
  • Neuronal electrical activity changes are linked to memory formation.
  • In vitro studies require methods for isolating individual neurons.

Purpose of the Study:

  • To develop a novel system for isolating and localizing individual neurons.
  • To enable in vitro studies of single-neuron electrical activity.
  • To facilitate the formation of neuronal networks.

Main Methods:

  • Designed and fabricated a 4x4 multiple microelectrode array system.
  • Utilized dielectrophoretic traps with gradient alternating current (AC) fields for neuron manipulation.
  • Employed three-dimensional finite element modeling (FEM) for electric field characterization.

Related Experiment Videos

  • Separated neurons from glial cells and positioned them on electrodes.
  • Recorded extracellular electrical activity from single neurons.
  • Applied fast Fourier transformation (FFT) analysis to determine burst rates.

    • Main Results:

    • Successfully spatially arranged neurons using dielectrophoretic traps.
    • Demonstrated the separation of neurons from glial cells.
    • Positioned individual neurons over single electrodes on the microelectrode array.
    • Recorded and analyzed the extracellular electrical activity of single neurons.
    • Determined characteristic burst rates of individual neurons via FFT analysis.

    Conclusions:

    • The developed microelectrode array system effectively isolates and localizes individual neurons.
    • Dielectrophoretic AC fields are a viable method for neuron manipulation and network formation.
    • This technology advances in vitro neuronal studies and the understanding of neuronal electrical activity.