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Related Experiment Videos

SKIP modifies gene expression by affecting both transcription and splicing.

Keisuke Nagai1, Tomohiro Yamaguchi, Tomonori Takami

  • 1Department of Life Science, Graduate School of Science, Himeji Institute of Technology, 3-2-1 Koto, Kamigori, Hyogo 678-1297, Japan.

Biochemical and Biophysical Research Communications
|March 17, 2004
PubMed
Summary

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SKIP (Ski-interacting protein) regulates gene expression through both transcriptional activation and pre-mRNA splicing modulation. This study clarifies its dual role in gene regulation.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Splicing

Background:

  • SKIP (Ski-interacting protein) is known as both a transcriptional coregulator and a spliceosome component.
  • The precise relationship between SKIP's transcriptional and splicing functions remains unclear.

Purpose of the Study:

  • To investigate the dual role of SKIP in gene expression.
  • To elucidate the connection between SKIP's functions in transcription and pre-mRNA splicing.

Main Methods:

  • Reporter gene assays were used to assess SKIP's effect on transcription from viral promoters.
  • Experiments involved reporter constructs with varying numbers of GC boxes to study promoter activity.
  • SKIP's impact on aberrant splicing at cryptic splice sites and a beta-thalassemia mutation was analyzed.

Related Experiment Videos

  • Transfection experiments compared gene expression levels of intron-containing and intronless reporters.

    • Main Results:

    • SKIP activated reporter gene expression from basal viral promoters, with a more pronounced effect on stronger promoters.
    • SKIP suppressed aberrant splicing at a cryptic splice donor site in a luciferase reporter.
    • SKIP also suppressed splicing of an introduced intron in the human beta-globin gene.
    • While intronless reporters showed higher expression, SKIP's activation was less significant compared to intron-containing reporters.

    Conclusions:

    • SKIP functions as a dual regulator of gene expression.
    • SKIP enhances gene expression through transcriptional activation.
    • SKIP also modulates gene expression by regulating pre-mRNA splicing processes.