Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developing a genetic system in Deinococcus radiodurans for analyzing mutations.

Mandy Kim1, Erika Wolff, Tiffany Huang

  • 1Department of Microbiology, Immunology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles 90095, USA.

Genetics
|March 17, 2004
PubMed
Summary

This study adapted the rifampicin resistance (Rif(r)) system from Escherichia coli to Deinococcus radiodurans, revealing mutation hotspots in the rpoB gene. These findings enhance our understanding of DNA repair mechanisms in radiation-resistant bacteria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-Sectional Survey of Unmet Needs in Children With NF1 and Cutaneous Neurofibromas: The Role of Pediatric Dermatology.

Pediatric dermatology·2026
Same author

Targeting cellular senescence in dermatology: senolytic and senomorphic strategies.

GeroScience·2026
Same author

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same author

Fast-conducting mechanonociceptors uniquely engage reflexive and affective pain circuitry to drive protective responses.

Neuron·2026
Same author

Strain sharing and persistence of microbial pathogens colonizing the skin of residents in a regional nursing home network.

Nature communications·2026
Same author

Response to 'On using clustering statistics for assessing plasmid binning tools accuracy'.

Briefings in bioinformatics·2026

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Deinococcus radiodurans exhibits extreme resistance to radiation-induced mutagenesis.
  • Understanding mutation mechanisms in D. radiodurans is crucial for comprehending its resilience.
  • Genetic tools for analyzing mutations in D. radiodurans are less developed compared to model organisms like E. coli.

Purpose of the Study:

  • To adapt and apply the rpoB/Rif(r) genetic system for analyzing base substitution mutations in Deinococcus radiodurans.
  • To identify mutation hotspots within the rpoB gene in D. radiodurans.
  • To compare mutation patterns between D. radiodurans and Escherichia coli.

Main Methods:

  • Adaptation of the rpoB/Rif(r) system, previously developed for E. coli, to D. radiodurans.

Related Experiment Videos

  • Sequencing of over 250 Rif(r) mutations arising spontaneously and after treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and 5-azacytidine (5AZ).
  • Analysis of mutations in both wild-type and uvrD (mismatch-repair-deficient) D. radiodurans backgrounds.

    • Main Results:

    • The rpoB/Rif(r) system successfully monitored base substitutions in D. radiodurans, defining 33 base change substitutions at 22 different base pairs.
    • The mutagenic specificities of NTG and 5AZ in D. radiodurans were consistent with those observed in E. coli and other organisms.
    • Prominent base substitution hotspots were identified in the rpoB gene of D. radiodurans, with some differing from E. coli hotspots despite sequence similarity.

    Conclusions:

    • The rpoB/Rif(r) system is a viable tool for studying base substitution mutations in D. radiodurans.
    • D. radiodurans possesses distinct mutation hotspots in its rpoB gene, offering insights into its unique DNA repair capabilities.
    • Comparative analysis reveals both conserved and divergent mutation patterns between D. radiodurans and E. coli.