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Updated: Aug 25, 2026

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
Novel expression patterns of phosphatidylinositol 3-hydroxy kinase in nodulated Medicago spp. plants
Luis E Hernández1, Carolina Escobar, Bjørn K Drøbak
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA Wageningen,The Netherlands. luisehernandez@uam.es
Abstract:
A cDNA clone encoding a phosphatidylinositol 3-kinase (PtdIns 3-kinase) has been characterized from Medicago truncatula, Mtpi3k, that is highly homologous to their counterparts from soybean (over 84%). The results suggest the presence of at least two genes coding PtdIns 3-kinases in M. truncatula. Mtpi3k transcript levels increased in nodules, compared with non-infected roots. Strikingly, Mtpi3k mRNA accumulated in young elongating stems at higher levels than that observed in other organs. Enhanced transcription of genes coding PtdIns 3-kinases might occur in tissues experiencing a high degree of vesicle trafficking and cell elongation.
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