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A streptavidin-biotin-based microarray platform for immunoassays

Petra Pavlickova1, Hubert Hug

  • 1Section of Experimental Anesthesiology, University Clinic Ulm, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 17, 2004
PubMed

The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.

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