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A new fluorescent cholesterol derivative: structure and blood plasma analysis
M A Abubaker1, R von Wandruszka
1Department of Chemistry, University of Idaho, Moscow 83843.
Photochemistry and Photobiology
|August 1, 1992
Abstract:
A fluorescent cholesterol derivative produced by reaction with gaseous HCl and zinc chloride in ethyl acetate is shown to be a chlorine substituted B-ring diene. The species forms relatively rapidly via an i-steroid rearrangement, requiring a temperature around 70 degrees C. A weak, possibly dimeric pi-allyl zinc complex exists in solution, leading to a red shift in the fluorescence emission. The application of the derivative to the determination of cholesterol in bovine plasma provides good sensitivity and precision and requires notably small sample volumes.