Related Experiment Video
Updated: Jul 1, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
Published on: December 12, 2013
13C nuclear overhauser polarization-magic-angle spinning nuclear magnetic resonance spectroscopy in uniformly
Etsuko Katoh1, K Takegoshi, Takehiko Terao
1Biochemistry Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Abstract:
A recently introduced (13)C polarization technique based on the nuclear Overhauser effect in rotating solid (nuclear Overhauser polarization-magic-angle spinning, NOP-MAS) (Takegoshi, K.; Terao, T. J. Chem. Phys. 2002, 117, 1700-1707) is applied to uniformly (13)C, (15)N-labeled proteins. NOP enhancement factors per scan of 1.5 approximately 2.0 are obtained, while that by cross polarization (CP) is less than 1.0. We show that uniform enhancement of all (13)C signals by CP is difficult to attain, while it is easily achieved by NOP, thus enabling quantitative comparison of signal intensities. NOP is easy to carry out under fast MAS and works well even for somewhat mobile molecules, for which CP does not work. Moreover, in labeled protein samples containing nonlabeled additives, NOP can eliminate the latter signals. For these features, NOP is superior to CP in many uniformly (13)C labeled proteins.
More Related Videos
12:47Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
14:55Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
Related Concept Videos
Carbon-13 (¹³C) NMR: Overview
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Atomic Nuclei: Magnetic Resonance
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
NMR Spectroscopy Of Amines