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Analysis of protein absorbed by LDL column (Liposorber) with special reference to complement component factor D
Osamu Oda1, Takashi Nagaya, Hiroshi Ogawa
1The Biodynamics Research Institute, 1-3-2 Tamamizu-cho, Mizuho-ku, Nagoya 467-8633, Japan.
Summary
The Liposorber column effectively removes LDL and also absorbs complement factor D in patients with chronic renal failure and arteriosclerosis obliterans. This finding highlights its broad efficacy in managing complex cardiovascular conditions.
Area of Science:
- Biochemistry
- Medical Technology
- Nephrology
Background:
- Dextran sulfate column (Liposorber) is effective for LDL apheresis in hyperlipemia.
- Liposorber shows positive outcomes in chronic renal failure (CRF) patients with arteriosclerosis obliterans (ASO).
Purpose of the Study:
- To analyze proteins absorbed by Liposorber in CRF patients with ASO.
- To identify and characterize specific proteins removed during LDL apheresis.
Main Methods:
- Proteomic analysis using 2D electrophoresis.
- Protein purification via Sephacryl S-100 and DEAE Sepharose fast flow chromatography.
- Identification of purified protein using Western blotting.
Main Results:
- Noteworthy protein spots were identified in absorbed fractions.
- A specific protein was purified and identified as complement factor D.
- An average of 6.5 mg of complement factor D was recovered, representing approximately 21.7% of the estimated plasma amount.
Conclusions:
- Liposorber demonstrates significant absorption of complement factor D.
- This suggests Liposorber's potential role beyond LDL removal in specific patient populations.