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Published on: May 15, 2019
Analysis of protein absorbed by LDL column (Liposorber) with special reference to complement component factor D
Osamu Oda1, Takashi Nagaya, Hiroshi Ogawa
1The Biodynamics Research Institute, 1-3-2 Tamamizu-cho, Mizuho-ku, Nagoya 467-8633, Japan.
Insights
The Liposorber column effectively removes LDL and also absorbs complement factor D in patients with chronic renal failure and arteriosclerosis obliterans. This finding highlights its broad efficacy in managing complex cardiovascular conditions.
Area of Science:
- Biochemistry
- Medical Technology
- Nephrology
Background:
- Dextran sulfate column (Liposorber) is effective for LDL apheresis in hyperlipemia.
- Liposorber shows positive outcomes in chronic renal failure (CRF) patients with arteriosclerosis obliterans (ASO).
Purpose of the Study:
- To analyze proteins absorbed by Liposorber in CRF patients with ASO.
- To identify and characterize specific proteins removed during LDL apheresis.
Main Methods:
- Proteomic analysis using 2D electrophoresis.
- Protein purification via Sephacryl S-100 and DEAE Sepharose fast flow chromatography.
- Identification of purified protein using Western blotting.
Main Results:
- Noteworthy protein spots were identified in absorbed fractions.
- A specific protein was purified and identified as complement factor D.
- An average of 6.5 mg of complement factor D was recovered, representing approximately 21.7% of the estimated plasma amount.
Conclusions:
- Liposorber demonstrates significant absorption of complement factor D.
- This suggests Liposorber's potential role beyond LDL removal in specific patient populations.
Background:
A dextran sulfate column (Liposorber) has been developed and proven effective in LDL apheresis for removing LDL from patients with hyperlipemia. Chronic renal failure (CRF) patients with arteriosclerosis obliterans (ASO) treated with Liposorber have been shown good results.
Methods:
We analyzed proteins absorbed by Liposorber in CRF patients with ASO by means of 2-dimensional electrophoresis and found some noteworthy protein spots. One of these proteins was purified by Sephacryl S-100 column and DEAE Sepharose fast flow column chromatography.
Results:
The purified protein was finally identified as complement factor D by Western blotting. The average of recovery amount from three experiments was 6.5 mg. The total recovered amount of factor D was calculated to be about 21.7% out of the estimated amount of factor D in the plasma of CRF patients with ASO receiving LDL apheresis using Liposorber.
Conclusions:
These results indicate that Liposorber absorbs factor D remarkably well.
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