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Two ITS forms co-inhabiting a single genet of an isolate of Terfezia boudieri (Ascomycotina), a desert truffle
Sharon Aviram1, Nurit Roth-Bejerano, Varda Kagan-Zur
1The Institutes for Applied Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Abstract:
Two fruit-bodies of Terfezia boudieri Chatin, each exhibiting a mixture of two ITS -RFLP profiles, were found in the Negev desert of Israel. A mycelial culture obtained from glebal out-growth maintained the double profile, as did proliferating cultures established using single hyphae isolated from the original cultures. The main difference between the two ITS variants lies in a 21 bp deletion in the smaller variant. The question whether both variants are contained within a single nucleus or occupy different nuclei sharing the same cytoplasm is discussed.
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