Related Experiment Video
Updated: Aug 25, 2026

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Published on: January 22, 2021
Effect of the antibacterial activity of pig pancreatic juice on human multiresistant bacteria
Danuta Kruszewska1, Asa Ljungh, Sean O Hynes
1Department of Medical Microbiology, Dermatology and Infection, Lund University, Lund, Sweden.
Introduction:
The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the current study was to assess the effect of pancreatic fluid on the growth of pathogenic bacteria and fungi.
Methods:
The antibacterial activity of pure pig pancreatic juice collected from catheterized, healthy, conscious, and anesthetized pigs was investigated with multiresistant microbial isolates and nonpathogenic strains. Studies were performed on pathogenic bacterial and fungi as well as lactic acid bacteria and reference strains.
Results:
Pancreatic juice was effective (P < 0.01) against multidrug resistant bacterial pathogens, whereas lactic acid bacteria were insensitive. The antibacterial action was independent of pancreatic juice proteolytic activity. The in vitro antibacterial properties of pancreatic juice last for several hours. Data suggest that broth composition may modulate the intensity of pancreatic juice antibacterial activity.
Conclusions:
Pancreatic juice antibacterial activity may be an important factor in limiting the colonization of pathogenic bacteria in the gastrointestinal tract. We postulate that observed antibacterial activity of the pancreatic juice could play an important role as one of the factors of innate immunity.
More Related Videos
09:17A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
Published on: January 2, 2017
07:46Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
Published on: June 5, 2012
Related Concept Videos
Clinical Significance of Antibiotic Resistance
Antimicrobial Effectiveness
Mechanism of Antibiotic Resistance in MRSA
Development of Antibiotic Resistance
Microbiota of the Stomach and Small Intestine
Microbiota Modulation by Antibiotics