Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003
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Junhui Zhai1, Thomas Briese, Erhei Dai
1Institute of Microbiology and Epidemiology, Academy of Military Sciences, Beijing, China.
Emerging Infectious Diseases
|March 20, 2004
Abstract:
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
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02:57Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
01:32PCR - Polymerase Chain Reaction
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