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Updated: May 28, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
[Genetic diversity of rare and endangered plant Sorbus amabilis]
Dengyi Liu1, Hao Shen, Yuehong Yang
1Biodiversity Research Center, Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources in Anhui, Anhui Normal University, Wuhu 241000, China. ldy@mail.ahnu.edu.cn
Abstract:
The genetic diversity of 13 natural populations of Sorbus amabilis Cheng ex Yü from different distribution regions was investigated using RAPD. Fourteen primers were screened from 40 ten-bp arbitrary primers, and a total of 105 DNA fragments were amplified among which, 30(28.6%) were polymorphic. DNA molecular dendrograms were established for the 13 natural populations, based on UPGMA and Neighbor-Joining. The result showed that S. amabilis had a low genetic diversity, and its adaptability to environmental change was weak. The genetic diversity of S. amabilis among the 13 natural populations were primarily related to their geographic distribution. Its special evolutionary history, man-made destruction, natural disaster (fire, plant diseases and insect pests etc.) and genetic drift caused by small population were the main reasons for the low-level genetic diversity of S. amabilis and its endangered position.
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