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Related Experiment Videos

Gene expression analysis for high throughput screening applications.

Albert Pinhasov1, Jay Mei, Dhammika Amaratunga

  • 1Johnson & Johnson Pharmaceutical Research mapsto Development, Spring House, PA 19477, USA.

Combinatorial Chemistry & High Throughput Screening
|March 23, 2004
PubMed
Summary

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We developed an automated high throughput TaqMan RT-PCR method for accurate mRNA expression analysis. This powerful tool enables reliable quantification of low-expression genes in drug discovery.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Growing demand for high throughput gene expression profiling.
  • Need for accurate and reproducible mRNA quantification methods.

Purpose of the Study:

  • Establish and validate a new automated high throughput TaqMan RT-PCR method.
  • Enable quantitative mRNA expression analysis for drug discovery applications.

Main Methods:

  • Utilized the Allegro System (Zymark) for automated sample tracking and liquid handling.
  • Employed the ABI PRISM 7900 HT Sequence Detection System for real-time PCR.
  • Integrated robotic loading and continuous wavelength detection for multiplexing.

Main Results:

Related Experiment Videos

  • Demonstrated the accuracy, reproducibility, and high throughput capability of the method.
  • Showcased its effectiveness in measuring low concentrations of mRNA.
  • Validated its reliability for quantifying low-expression genes.

    • Conclusions:

    • The automated high throughput TaqMan RT-PCR is a powerful tool for quantitative mRNA analysis.
    • It supports target identification/validation, SAR studies, compound selection, and biomarker identification.
    • This method is reliable for drug discovery and development processes.