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Related Concept Videos

Proteomics01:33

Proteomics

9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Related Experiment Video

Updated: Jan 9, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

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Exploring the phosphoproteome with mass spectrometry.

Eric C Peters1, Ansgar Brock, Scott B Ficarro

  • 1Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA. epeters@gnf.org

Mini Reviews in Medicinal Chemistry
|March 23, 2004
PubMed
Summary

Mass spectrometry advances enable broader analysis of protein phosphorylation, a key regulatory process. This technique helps understand complex cellular signaling networks through post-translational modification assessment.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein phosphorylation is a vital reversible post-translational modification controlling cellular regulatory pathways.
  • Understanding complex, interconnected signaling networks is essential for biological research.
  • Existing analysis techniques may not fully capture the scope of phosphorylation events.

Purpose of the Study:

  • To summarize recent advancements in mass spectrometry for analyzing phosphorylation.
  • To highlight the application of these techniques in increasingly complex biological systems.
  • To provide an overview of broader-scale analysis methods for phosphorylation.

Main Methods:

  • Mass spectrometry-based proteomics.
  • Advanced analytical techniques for post-translational modification analysis.
  • High-throughput screening of phosphorylation sites.

Main Results:

  • Recent progress has expanded the scale and depth of phosphorylation analysis using mass spectrometry.
  • These techniques allow for the assessment of phosphorylation events in more complex biological contexts.
  • New methods facilitate a more comprehensive understanding of phosphorylation networks.

Conclusions:

  • Mass spectrometry is a powerful tool for advancing the study of protein phosphorylation.
  • Broader-scale analysis techniques are crucial for deciphering complex regulatory networks.
  • Continued development in mass spectrometry will enhance our understanding of cellular signaling.