Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Physical and functional interactions between Daxx and TSG101.

Ryuta Muromoto1, Kenji Sugiyama, Tetsuya Yamamoto

  • 1Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo 060-0812, Japan.

Biochemical and Biophysical Research Communications
|March 23, 2004
PubMed
Summary

Daxx protein interacts with TSG101 in the nucleus, forming a complex that enhances transcriptional repression. This finding reveals a novel interaction for Daxx (Death-associated protein 6) and TSG101 (Tumor Susceptibility Gene 101) in gene regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Hemoglobin Improvement on Survival in Patients With Myelofibrosis and Anemia Treated With Momelotinib in the SIMPLIFY-1 and MOMENTUM Trials.

European journal of haematology·2026
Same author

Case Report: Dramatic metabolic improvement with tirzepatide in a patient with acquired partial lipodystrophy following hematopoietic stem cell transplantation.

Frontiers in endocrinology·2026
Same author

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same author

The tumor suppressor NDRG2 recruits protein phosphatase 2A to suppress STAT5 phosphorylation in adult T-cell leukemia/lymphoma.

The Journal of biological chemistry·2026
Same author

Cathepsin G is associated with cerebral vascular injury in myeloid leukemia: a pathologic insight into intracranial hemorrhage.

Research and practice in thrombosis and haemostasis·2026
Same author

Efficacy and safety of a three-step dose escalation regimen of ropeginterferon alfa-2b in Japanese patients with polycythemia vera: a phase 3b, single-arm, multicenter study.

International journal of hematology·2026

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Daxx (Death-associated protein 6) is known to mediate cytoplasmic signals but also exhibits nuclear functions as a transcriptional regulator.
  • TSG101 (Tumor Susceptibility Gene 101) is recognized as a transcriptional co-repressor for nuclear hormone receptors.

Purpose of the Study:

  • To investigate the direct interaction between Daxx and TSG101.
  • To determine the cellular localization and functional consequences of the Daxx-TSG101 interaction.

Main Methods:

  • Yeast two-hybrid screening to identify Daxx binding partners.
  • Co-immunoprecipitation assays using co-expressed tagged proteins to confirm Daxx-TSG101 association.
  • Mapping of interaction regions within Daxx and TSG101.

Related Experiment Videos

  • Analysis of cellular localization and impact on transcriptional activity.

    • Main Results:

    • Direct interaction between Daxx and TSG101 was confirmed.
    • The interaction involves TSG101's coiled-coil domain and occurs within the nucleus.
    • TSG101 binding enhances Daxx-mediated repression of glucocorticoid receptor transcriptional activity.

    Conclusions:

    • A novel molecular interaction between Daxx and TSG101 has been established.
    • The Daxx-TSG101 complex functions as an efficient repressive transcription complex in the nucleus.
    • This interaction provides new insights into the nuclear roles of Daxx in transcriptional regulation.