Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD14 is an acute-phase protein.

Sylvette Bas1, Benoit R Gauthier, Ursula Spenato

  • 1Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland. sylvette.bas@hcuge.ch

Journal of Immunology (Baltimore, Md. : 1950)
|March 23, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of clinical criteria for the definition of disease activity in adult-onset Still's disease: a cohort study.

The Lancet. Rheumatology·2026
Same author

IL1R2 Deficiency Unleashes Neutrophil-Mediated Antitumor Potential in Sarcoma.

Cancer immunology research·2026
Same author

[Rheumatoid arthritis pathogenesis. From susceptibility to autoimmunity, and arthritis].

Revue medicale suisse·2026
Same author

Pyrin inflammasome activation triggers an IL-18-driven IFN-γ response in mevalonate kinase deficiency.

The Journal of allergy and clinical immunology·2026
Same author

A Multifaceted Interplay Among Hemophagocytosis, Interleukin-18, and Type I Interferon Distinguishes Still Disease From Other Autoinflammatory Diseases.

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

Cancer cell-intrinsic inflammasome protein ASC links innate immunity with mitochondrial metabolism in driving pancreatic cancer.

Nature communications·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

TGF-β controls developmental fate and functional identity of thymic γδ T cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Distinguishing Th17 cells as a unique subset.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Soluble CD14 (sCD14) is identified as a type 2 acute-phase protein (APP). Its production is regulated by IL-6, particularly in liver cells, and correlates with disease activity in arthritis patients.

Area of Science:

  • Immunology
  • Biochemistry
  • Hepatology

Background:

  • The origin and regulation of soluble CD14 (sCD14) in circulation remain unclear.
  • Investigating CD14's role as an acute-phase protein (APP) is crucial for understanding inflammatory responses.

Purpose of the Study:

  • To determine if CD14 functions as an acute-phase protein (APP).
  • To investigate the regulation of CD14 synthesis, particularly by IL-6 in liver cells.

Main Methods:

  • ELISA measurement of sCD14, IL-6, and C-reactive protein in patient serum and synovial fluid.
  • In vitro studies using HepG2 hepatoma cells and primary hepatocytes to assess CD14 production.
  • In vivo experiments in IL-6 knockout mice to confirm the role of IL-6 in CD14 regulation during acute-phase response.

Related Experiment Videos

Main Results:

  • Serum sCD14 levels correlated with C-reactive protein and IL-6 levels, and disease activity in arthritis.
  • IL-6 stimulation increased CD14 production at both protein and mRNA levels in liver cells.
  • Liver CD14 mRNA levels increased during acute-phase response in wild-type mice but not in IL-6 knockout mice.

Conclusions:

  • Soluble CD14 (sCD14) is identified as a type 2 acute-phase protein (APP).
  • IL-6 plays a key role in regulating sCD14 synthesis in the liver.
  • sCD14 levels reflect disease activity in inflammatory conditions like rheumatoid arthritis and reactive arthritis.