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Published on: August 6, 2019
3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis
J Robin Harris1, Ulrich Meissner, Wolfgang Gebauer
1Institute of Zoology, Johannes Gutenberg University, D-55099, Mainz, Germany.
Abstract:
Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a approximately 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1.

