A novel IS element, ISMpa1, in Mycobacterium avium subsp. paratuberculosis

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Ingrid Olsen1, Tone Bjordal Johansen, Helen Billman-Jacobe

  • 1Department of Animal Health, National Veterinary Institute, Pb 8156 Dep., 0033 Oslo, Norway. ingrid.olsen@vetinst.no

Veterinary Microbiology
|March 24, 2004
PubMed

Insights

A novel insertion element, ISMpa1, was discovered in Mycobacterium avium subsp. paratuberculosis. This element aids in classifying M. avium subsp. avium and identifying M. avium subsp. paratuberculosis strains.

Area of Science:

  • Microbiology
  • Genetics

Background:

  • Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease.
  • Distinguishing MAP from closely related species like Mycobacterium avium subsp. avium is crucial for diagnostics and epidemiology.

Purpose of the Study:

  • To identify and characterize novel genetic elements in MAP.
  • To evaluate the potential of these elements for strain typing and diagnostics.

Main Methods:

  • Whole-genome analysis of M. avium subsp. paratuberculosis.
  • Identification and sequencing of insertion sequence (IS) elements.
  • Restriction Fragment Length Polymorphism (RFLP) analysis using IS element probes.

Main Results:

  • A novel IS110 family insertion element, ISMpa1, was identified in the MAP genome.
  • ISMpa1 was present in all examined MAP strains but absent in most other mycobacteria, with exceptions in specific M. avium subsp. avium strains.
  • ISMpa1-based RFLP provided distinct patterns for MAP and positive M. avium subsp. avium strains.

Conclusions:

  • ISMpa1 is a potentially valuable marker for the identification of M. avium subsp. paratuberculosis.
  • ISMpa1 can be used in conjunction with IS900 for improved diagnostic accuracy.
  • The element may also serve as a tool for classifying strains within Mycobacterium avium subsp. avium.

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