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Two-dimensional electrophoretic protein profile analysis following exposure of human uroepithelial cells to
Mary Helen Kanitz1, Santhanam Swaminathan, Russell E Savage
1Division of Applied Research and Technology, Taft Laboratory, National Institute for Occupational Safety and Health, MS/C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA. mhk2@cdc.gov
Abstract:
Protein biomarkers to occupational carcinogens were investigated using a transformable human uroepithelial cell system, SV-HUC.PC. SV-HUC.PC was treated with N-hydroxy-4,4'-methylene bis (2-chloroaniline) (N-OH-MOCA) or N-hydroxy-4 aminobiphenyl (N-OH-ABP). Two-dimensional gel electrophoresis of cell lysates compared protein changes across treatments. Increasing N-OH-MOCA resulted in a dose-related increase in protein spots altered. Comparing cell profiles treated with either carcinogen revealed alterations in the expression of nine proteins, identified using the TagIdent database. These demonstrated isoelectric point shift (1) or quantity change (8). Our investigation may be useful in identifying biomarkers of effects of exposure to bladder carcinogens.
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