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Related Experiment Videos

Do high progesterone concentrations decrease pregnancy rates in embryo recipients synchronized with PGF2alpha and

Marcelo F Gouveia Nogueira1, Danilas S Melo, Luciano M Carvalho

  • 1Department of Pharmacology, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.

Theriogenology
|March 24, 2004
PubMed
Summary

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Equine chorionic gonadotropin (eCG) treatment in heifers increased progesterone levels but did not improve embryo transfer pregnancy rates. Higher eCG doses correlated with decreased conception rates, suggesting potential negative impacts.

Area of Science:

  • Veterinary Medicine
  • Reproductive Biology
  • Endocrinology

Background:

  • Embryo transfer (ET) is a key reproductive technology in cattle.
  • Optimizing recipient cyclicity and endocrine status is crucial for successful ET.
  • Equine chorionic gonadotropin (eCG) is used to manipulate ovarian function.

Purpose of the Study:

  • To investigate the effect of varying doses of eCG on accessory corpus luteum (CL) induction, progesterone levels, and pregnancy rates in cross-bred heifers undergoing embryo transfer.
  • To determine the optimal eCG dosage for enhancing pregnancy outcomes in ET recipients.

Main Methods:

  • Heifers received PGF2alpha, followed by estrus detection and randomization into four groups (control, 200, 400, 600 IU eCG).
  • Estrus was re-induced, and recipients received frozen-thawed embryos if they had multiple CL or a large single CL.

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  • Plasma progesterone concentrations were measured, and pregnancy was diagnosed via ultrasonography and confirmed by palpation.

    • Main Results:

    • eCG treatment significantly increased plasma progesterone concentrations in a dose-dependent manner.
    • The 200 IU eCG group showed a numerically higher conception rate (50.0%) compared to the control (41.9%), but this was not statistically significant.
    • Conception rates significantly decreased in the 400 IU (25.0%) and 600 IU (20.9%) eCG groups compared to the control and 200 IU groups.

    Conclusions:

    • While eCG treatment elevates progesterone, it does not enhance pregnancy rates in ET recipients.
    • Higher doses of eCG (400 and 600 IU) negatively impacted conception rates, indicating a potential detrimental effect at supra-physiological levels.
    • Further research is needed to elucidate the mechanisms behind the decreased conception rates observed with higher eCG doses.